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Guide for SCYM: Specialist in Cytometry

Quiz SCYM: Specialist in Cytometry

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Quiz

1/10
Instrumentation
In a conventional cuvette-based cytometer, the sample core is confined to the center of the flow cell so cells pass single file through the laser. Which physical condition is primarily responsible for this confinement?
Select the answer
1 correct answer
A.
Turbulent mixing of sheath and sample fluid at the injector tip
B.
Electrostatic repulsion between the charged cell membrane and the sheath
C.
Laminar flow of a faster sheath stream surrounding a slower, lower-volume sample core
D.
Centrifugal force generated by the tapered nozzle geometry

Quiz

2/10
Panel/Experiment Design
A 2 mL cerebrospinal fluid specimen is collected from a patient with suspected central nervous system involvement by diffuse large B-cell lymphoma. The courier will take about 45 minutes to reach the flow cytometry laboratory. Which handling approach best preserves the diagnostic yield of this specimen?
Select the answer
1 correct answer
A.
Centrifuge the fluid at the bedside and send only the dry cell pellet on wet ice
B.
Add the fluid to a protein-containing stabilizing medium such as serum-supplemented RPMI and transport at room temperature for immediate processing
C.
Add liquid EDTA to the fluid and refrigerate it overnight so it can be batched with the next morning's run
D.
Fix the fluid in 1 percent formaldehyde immediately after collection to prevent any cell loss

Quiz

3/10
Applications
On a CD45 versus side scatter display of a bone marrow aspirate, which population occupies the region used as the primary blast gate?
Select the answer
1 correct answer
A.
Mature lymphocytes, which show bright CD45 and low side scatter
B.
Granulocytes, which show high side scatter and moderate CD45
C.
Blasts, which show dim CD45 and low to intermediate side scatter
D.
Monocytes, which show the brightest CD45 and intermediate side scatter

Quiz

4/10
Data
A laboratory receives an FCS 3.1 file from a referring center and needs to determine which fluorochromes were assigned to each detector. Which segment of the file holds this information?
Select the answer
1 correct answer
A.
The HEADER segment, because it lists the parameter names and stain assignments
B.
The DATA segment, because the event values carry their own labels
C.
The TEXT segment, because it stores the ASCII keyword and value pairs such as $PnN and $PnS
D.
The ANALYSIS segment, because parameter identity is only defined once gates are applied

Quiz

5/10
Laboratory Operations
Daily QC on a four-laser analyzer uses a single multi-peak fluorescent microsphere lot. Over one run the bright peak median values are within target for every detector, but the robust CV of the bright peak has risen from 2.1 percent to 6.8 percent in all detectors on all lasers simultaneously. What is the most likely explanation?
Select the answer
1 correct answer
A.
One photomultiplier tube has lost gain and needs a higher voltage
B.
The bead lot has expired and the assigned target values no longer apply
C.
A fluidics problem such as a partial clog or air in the sheath line is destabilizing the core stream
D.
The optical filter in front of the affected detector has degraded

Quiz

6/10
Instrumentation
A technologist increases the sample flow rate setting from low to high while acquiring a DNA ploidy specimen. What is the expected effect on the core stream and on the measured CV of the G0/G1 peak?
Select the answer
1 correct answer
A.
The core stream widens and the CV of the G0/G1 peak increases
B.
The core stream narrows and the CV improves
C.
The core diameter is unchanged and only the event rate rises
D.
The core widens but the CV improves because more photons are collected

Quiz

7/10
Panel/Experiment Design
A bone marrow aspirate submitted for lymphoma staging yields a population profile dominated by mature lymphocytes and segmented neutrophils, with almost no CD34 positive precursors, no hematogones, and no immature granulocytic forms. The profile is nearly identical to the peripheral blood drawn from the same patient the same morning. Which conclusion is best supported?
Select the answer
1 correct answer
A.
The marrow is aplastic and no further aspiration attempts should be made
B.
The specimen was collected in the wrong anticoagulant and must be rejected outright
C.
The aspirate is substantially hemodiluted with peripheral blood and may not represent the marrow compartment
D.
The specimen was overfixed during transport, which selectively destroyed precursor cells

Quiz

8/10
Applications
A staging marrow is analyzed and CD13 versus CD16 is displayed on maturing granulocytic cells. Which description matches the expected normal maturation pattern?
Select the answer
1 correct answer
A.
CD13 is bright on promyelocytes, dips at the myelocyte stage while CD16 is still absent, and then both increase so that mature neutrophils are CD13 positive and CD16 bright, producing a curved trajectory
B.
CD13 and CD16 are expressed at uniformly high levels at every stage of granulocytic maturation
C.
CD16 is acquired at the promyelocyte stage and is progressively lost as neutrophils mature
D.
CD13 and CD16 are both absent until the band stage and then appear together at full intensity

Quiz

9/10
Data
A file lists $P4E as 4,0 and $P4R as 1024 for a fluorescence detector. How should a channel value of 512 in that parameter be interpreted?
Select the answer
1 correct answer
A.
The parameter was stored with logarithmic scaling covering 4 decades across 1024 channels, so channel 512 corresponds to 10 raised to the power of 4 times 512 divided by 1024, or 100 relative units
B.
The parameter is linear with a gain of 4, so channel 512 corresponds to a value of 2048
C.
The parameter has only 4 usable channels and a dynamic range of 1024 volts
D.
The value cannot be interpreted at all unless $P4G is also present in the file

Quiz

10/10
Laboratory Operations
A Levey-Jennings chart for the PMT voltage required to place the QC bead peak on its target channel shows a steady increase of roughly 6 volts per week over eight consecutive weeks in one violet-excited detector, with every individual point still inside plus or minus 2 standard deviations. What action is most appropriate?
Select the answer
1 correct answer
A.
Investigate now as a trend, checking violet laser power and the detector, and document the findings
B.
Take no action, because no point has exceeded the 2 standard deviation limit
C.
Immediately widen the acceptance limits to accommodate the new voltage range
D.
Recalculate the target channel from the last eight weeks of data and continue
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  • Quiz name:SCYM: Specialist in Cytometry
  • Total number of questions:300
  • Number of questions for the test:50
  • Pass score:80%

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